A9104

AKT3, active human

buffered aqueous glycerol solution, protein apparent mol wt ~83 kDa, ≥85% (SDS-PAGE), recombinant, expressed in E. coli

• Synonym(s): PKBγ, Protein Kinase B γ, RAC-gamma serine/threonine-protein kinase, STK-2
• MDL number: MFCD07370217
• UNSPSC Code: 12352200

Properties

• recombinant: expressed in E. coli
• assay: ≥85% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 300-3000 units/mg protein
• mol wt: protein apparent mol wt ~83 kDa
• UniProt accession no.: Q9Y243
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... AKT3(10000)

Description

Application

Kinase activity is measured as the molar amount of phosphate incorporated Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 °C using a final concentration of 50 μM [³²P]-ATP.

Biochem/physiol Actions

One of three highly conserved isoforms, which are designated in humans as PKBα (Akt1), PKBβ (Akt2), and PKBγ (Akt3). Akt3 is expressed widely, but not in liver or skeletal muscle, suggesting that its principle function may not be in regulating insulin signaling. Akt3 is involved in tumorigenesis and may contribute to the more aggressive clinical phenotype of the estrogen receptor-negative breast cancers and androgen-insensitive prostate carcinomas.

Unit Definition

One unit will incorporate 1 nanomole of phosphate into the AKT/SGK substrate peptide (RPRAATF) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm.

Physical form

Supplied at a concentration of approximately 0.1 mg/mL in 50 μl of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA and 30% glycerol

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable