M6542

Macrophage Inflammatory Protein-1β from mouse

recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): mMIP-1β, MIP-1β
• MDL number: MFCD00213006
• UNSPSC Code: 12352202

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE and N-terminal analysis)
• form: lyophilized powder
• potency: 40-100 ng/mL ED₅₀/EC₅₀
• mol wt: 7.8 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P14097
• storage temp.: −20°C
• Gene Information: mouse ... Ccl4(20303)

Description

General description

MIP-1α and MIP-1β (macrophage inflammatory protein) were originally co-purified from LPS stimulated mouse macrophages. Recombinant, mouse MIP-1β (rmMIP-1β) consists of 69 amino acids. MIP- 1β belongs to the chemokine β subfamily which is characterized by a C-C configuration at the first two cysteines. MIP-1β has endogenous pyrogenic activity when it is injected intravenously into rabbits. Although other cytokines, such as IL-1alpha, IL-1β and TNF have endogenous pyrogenic activity, the pyrogenic effects of these cytokines can be inhibited by cyclooxgenase blockers, while the pyrogenicity of MIP- 1β is unaffected by these agents. MIP-1β can synergize with the hematopoietic growth factors granulocyte macrophage CSF (GM-CSF) or macrophage CSF (M-CSF) to enhance colony formation.

Biochem/physiol Actions

MIP-1β has endogenous pyrogenic activity when it is injected intravenously into rabbits. MIP-1 can synergize with the hematopoietic growth factors granulocytemacrophage CSF (GM-CSF) or macrophage CSF (M-CSF) to enhance colony formation.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin

Analysis Note

The biological activity of MIP-1β was tested in culture by measuring its ability to inhibit hematopoietic stem cell proliferation in an in vitro colony assay.