NA2120

GenElute^™ Bacterial Genomic DNA Kits

sufficient for 350 purifications

• Synonym(s): Bacterial Genomic DNA, bacterial DNA prep, Gen Elute
• UNSPSC Code: 41105501
• NACRES: NA.55

Properties

• usage: sufficient for 350 purifications
• Quality Level: 200
• technique(s): DNA purification: suitable
• storage temp.: 15-25°C

Description

General description

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified bacterial genomic DNA is ready for downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• cloning

The purified bacterial genomic DNA is ready for downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• cloning

GenElute^™ Bacterial Genomic DNA Kits has been used:

• to isolate DNA
• to prepare DNA from E. coli strain
• to extract genomic DNA

Features and Benefits

• Starting material: Up to 1.5 mL of culture
• Expected yield: Up to 20 μg
• Elution volume: 400 μl
• Time required: 70 - 120 min
• A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
• No phenol, chloroform, or ethanol precipitation required

• Typical DNA yields of 15 μg - 20 μg
• Protocols provided for Gram + and Gram - bacteria
• High quality genomic DNA in less than 2 hours
• Purified DNA has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9

Principle

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• W0263 Wash Solution 1
• C2112 Column Preparation Solution
• P2308 Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
• R6148 RNase A solution

Safety Information

• pictograms: GHS02,GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H226,H290,H302,H315,H318,H334,H335,H336,H410
• pcodes: P210 - P273 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Central nervous system, Respiratory system
• Storage Class: 3 - Flammable liquids
• flash_point_f: 77.0 °F - closed cup
• flash_point_c: 25 °C - closed cup