Skip to Content
MilliporeSigma
All Photos(3)

Key Documents

NA2120

Sigma-Aldrich

GenElute Bacterial Genomic DNA Kits

sufficient for 350 purifications

Synonym(s):

Bacterial Genomic DNA, bacterial DNA prep, Gen Elute

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41105501
NACRES:
NA.55

Pricing and availability is not currently available.

usage

sufficient for 350 purifications

Quality Level

technique(s)

DNA purification: suitable

storage temp.

15-25°C

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
5512607778461.07293
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

mp

>252 °C (dec.) (lit.)

mp

237 °C (lit.)

mp

124-128 °C

mp

107-109 °C

functional group

sulfonic acid

functional group

carboxylic acid

functional group

-

functional group

-

General description

The GenElute Bacterial Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from gram-negative bacteria. For most gram-positive bacteria, the kit must be used in conjunction with the optional lysozyme (L4919) to effectively lyse the thick peptidoglycan cell walls. A Gram-Positive Lysis Solution is provided as a diluent for preparing the lysozyme stock solutions.

The kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning
The purified bacterial genomic DNA is ready for downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • cloning

GenElute Bacterial Genomic DNA Kits has been used:
  • to isolate DNA[1][2][3]
  • to prepare DNA from E. coli strain[4]
  • to extract genomic DNA[5]

Features and Benefits

  • Starting material: Up to 1.5 mL of culture
  • Expected yield: Up to 20 μg
  • Elution volume: 400 μl
  • Time required: 70 - 120 min
  • A260/A280 ratio: 1.6 - 1.9
  • No phenol, chloroform, or ethanol precipitation required
  • Typical DNA yields of 15 μg - 20 μg
  • Protocols provided for Gram + and Gram - bacteria
  • High quality genomic DNA in less than 2 hours
  • Purified DNA has an A260/A280 ratio between 1.6 and 1.9

Principle

The bacteria are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane into a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution.

The expected yield of genomic DNA will vary depending on the cell density of the bacterial culture and the bacterial species and strain used. DNA purified with the GenElute kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • W0263Wash Solution 1SDS

  • C2112Column Preparation SolutionSDS

  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS

  • R6148RNase A solutionSDS

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system, Respiratory system

Storage Class

3 - Flammable liquids

flash_point_f

77.0 °F - closed cup

flash_point_c

25 °C - closed cup


  • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Ayako Tokuda
    Kokubyo Gakkai zasshi. The Journal of the Stomatological Society, Japan, 71(1), 18-26 (2004-04-24)
    To fabricate a prosthesis which is functionally harmonized with the stomatognathic system, the interocclusal relationship must be reproduced as accurately as possible. This study investigated the influence of the occlusal contacts upon tooth displacement and clarified the optimal occlusal contacts
    Martin Schuettler et al.
    Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2008, 3212-3215 (2009-01-24)
    Standard interconnection technologies are reviewed in respect to their applicability to electrically and mechanically connect laser-patterned nerve electrodes made from silicone rubber and platinum foil to wires and screen printed alumina substrates. Laser welding, gap welding, microflex ball bonding, and
    Yo Shibata et al.
    The International journal of oral & maxillofacial implants, 19(2), 177-183 (2004-04-23)
    This study evaluated the quality of coatings on titanium surfaces prepared by discharging in Hanks' balanced salt solution without organic molecules (HBSS-). 10 x 10 x 1.0-mm titanium plates were used as cathodes of a coating device developed in the
    J A Sorensen et al.
    The Journal of prosthetic dentistry, 67(1), 16-22 (1992-01-01)
    This study evaluated the marginal fidelity and microleakage of porcelain veneers made with the platinum foil and refractory die techniques. Maxillary incisors, matched for size and amount of enamel, were prepared with 0.5 mm uniform intraenamel reduction. The indirectly made
    Ayumi Ikeda et al.
    Journal of medical and dental sciences, 52(4), 195-202 (2006-05-04)
    The purpose of this study was to clarify the influence of changes of occlusal contacts on adjacent tooth displacement during articulation. The amount and direction of displacement of the maxillary left first molar and adjacent teeth were measured using a

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service