Name: Sodium Bicarbonate
Brand: SIGMA

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About This Item

CAS Number:

144-55-8

MDL number:

MFCD00003528

UNSPSC Code:

12161702

PubChem Substance ID:

24899815

NACRES:

NA.75

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Product Name

Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

sterility

sterile-filtered

product line

BioReagent

form

solution (7.5%)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.8-8.2

web metadata keyword.default

Physiological buffer, pH adjustment reagent, carbon dioxide, neutral pH buffer

storage temp.

2-8°C

SMILES string

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

General description

Sodium bicarbonate (NaHCo₃) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.

Application

Sodium bicarbonate solution has been used:

as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice
as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants
as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma
to prepare collagen mix droplets for 3D collagen sprouting assay
to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture

Preparation Note

Prepared in cell culture grade water.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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