A6185

Calpain Inhibitor I

≥97% (TLC), powder

• Synonym(s): ALLN, Ac-LLnL-CHO, MG-101, N-Acetyl-L-leucyl-L-leucyl-L-norleucinal, N-Acetyl-Leu-Leu-Norleu-al
• Empirical Formula (Hill Notation): C₂₀H₃₇N₃O₄
• CAS Number: 110044-82-1
• Molecular Weight: 383.53
• Beilstein/REAXYS Number: 7656053
• MDL number: MFCD00065505
• UNSPSC Code: 12352200
• PubChem Substance ID: 24891066
• NACRES: NA.77

Properties

• biological source: synthetic (organic)
• Quality Level: 200
• assay: ≥97% (TLC)
• form: powder
• color: white
• solubility: DMSO: soluble; ethanol: soluble
• storage temp.: −20°C
• SMILES string: [H]C(=O)[C@H](CCCC)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(C)=O
• InChI: 1S/C20H37N3O4/c1-7-8-9-16(12-24)22-19(26)18(11-14(4)5)23-20(27)17(10-13(2)3)21-15(6)25/h12-14,16-18H,7-11H2,1-6H3,(H,21,25)(H,22,26)(H,23,27)/t16-,17-,18-/m0/s1
• InChI key: FMYKJLXRRQTBOR-BZSNNMDCSA-N
• Gene Information: human ... CAPN1(823), CTSB(1508), PSMA1(5682)

Description

Application

Calpain Inhibitor I have been used:

• as a component of protease inhibitor cocktail for gel shift analysis
• for treating HeLa cell cultures
• for inhibition of 26 S proteasome in 26 S proteasome inhibition assays

Biochem/physiol Actions

Calpain Inhibitor I inhibits tumor necrosis factor-induced (TNF-induced) cleavage of Poly (ADP-ribose) polymerase (PARP) and calpastatin in apoptosis. It also inhibits oxygen/glucose deprivation (OGD) induced cleavage of caspase-12 and Bcl-xL..

Can initiate apoptosis in HL-60 cells but blocks dexamethasone-induced apoptosis in thymocytes and cycloheximide-induced apoptosis in metamyelocytes. Inhibits cyclin B degradation and arrests the cell cycle at G₁/S and at meta-/anaphase. Blocks induction of nitric oxide synthase by LPS in macrophages.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)