B7435

CelLytic^™ B Cell Lysis Reagent

For bacterial cell lysis, standard strength

• Synonym(s): Cell lysis reagent
• MDL number: MFCD02097909
• UNSPSC Code: 41116134
• NACRES: NA.77

Properties

• Quality Level: 200
• form: solution

Description

Application

A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.

CelLytic^™ B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10, recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein and human α-synuclein.

Features and Benefits

• Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
• Scalable for 1 to 25 grams of bacterial cell paste
• No interference with downstream applications such as affinity chromatography, IP, and Western blotting
• Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents

Other Notes

Detergent blend formulated in 40 mM Trizma^® HCl (pH 8.0).

Legal Information

Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H319
• pcodes: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type ABEK (EN14387) respirator filter