C3306

Calcium chloride dihydrate

≥99.0%

• Synonym(s): CaCl₂.2H₂O, Calcium chloride, Calcium dichloride
• Empirical Formula (Hill Notation): CaCl₂ · 2H₂O
• CAS Number: 10035-04-8
• Molecular Weight: 147.01
• EC Number: 233-140-8
• MDL number: MFCD00149613
• UNSPSC Code: 12352302
• PubChem Substance ID: 24892603
• NACRES: NA.31
• assay: ≥99.0%
• grade: Molecular Biology
• form: powder
• solubility: H₂O: 294 mg/mL, clear, colorless

Properties

• grade: Molecular Biology
• Quality Level: 200
• assay: ≥99.0%
• form: powder
• mp: 176 °C (dec.) (lit.)
• solubility: H₂O: 294 mg/mL, clear, colorless
• foreign activity: DNase, RNase, protease, none detected
• SMILES string: O.O.Cl[Ca]Cl
• InChI: 1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
• InChI key: LLSDKQJKOVVTOJ-UHFFFAOYSA-L

Description

Application

Calcium chloride dihydrate has been used:
to prepare competent E. coli for transformation(118)
in the preparation of calcium polyphosphate nanospheres and to induce alkaline phosphatase(119)
as a component of renaturation buffer for the zymogram of Staphylococcus aureus crude enzyme extracts(120)

Biochem/physiol Actions

Calcium chloride is used in the heat-shock transformation of microbes with the plasmid. The positive charged calcium forms a cation bridge with phosphate of DNA and negative charged phosphorylated lipid A in lipopolysaccharide (LPS) aiding the DNA transformation into the microbes.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves