C5041

6-Carboxyfluorescein diacetate

~95% purity (HPLC), powder

• Synonym(s): 6-CFDA, 6-Carboxy-di-O-acetylfluorescein
• Empirical Formula (Hill Notation): C₂₅H₁₆O₉
• CAS Number: 3348-03-6
• Molecular Weight: 460.39
• Beilstein/REAXYS Number: 67043
• MDL number: MFCD00036871
• UNSPSC Code: 12171500
• PubChem Substance ID: 24892748
• NACRES: NA.47

Properties

• Product Name: 6-Carboxyfluorescein diacetate, ≥95% (HPLC)
• Quality Level: 100; 200
• assay: ≥95% (HPLC)
• form: powder
• color: white to yellow
• solubility: chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5ccc(cc45)C(O)=O)c1
• InChI: 1S/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)

Description

Application

6-Carboxyfluorescein diacetate has been used to differentiate viable cells from apoptotic cells. Live cells that are not apoptotic accumulate 6-carboxyfluorescein but do not accumulate annexin.
Fluorescent wavelengths λex 492nm; λemm 517nm in 0.1M Tris, pH 8.0.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)