Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

C7089

Sigma-Aldrich

Monoclonal Anti-Cdk7/CAK antibody produced in mouse

clone MO-1.1, ascites fluid

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00282167
UNSPSC Code:
12352203
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

MO-1.1, monoclonal

mol wt

antigen 40 kDa

contains

15 mM sodium azide

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:50,000 using a cultured human cell line extract

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
C6613C8218HPA002637
Monoclonal Anti-Cdk7/CAK antibody produced in mouse clone MO-1.1, ascites fluid

C7089

Monoclonal Anti-Cdk7/CAK antibody produced in mouse

Anti-Cdc7 Kinase antibody, Mouse monoclonal clone DCS-341, purified from hybridoma cell culture

C6613

Anti-Cdc7 Kinase antibody, Mouse monoclonal

Monoclonal Anti-Cdk4 antibody produced in mouse clone DCS-31, ascites fluid

C8218

Monoclonal Anti-Cdk4 antibody produced in mouse

Anti-CDK6 antibody produced in rabbit affinity isolated antibody, buffered aqueous glycerol solution

HPA002637

Anti-CDK6 antibody produced in rabbit

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

clone

MO-1.1, monoclonal

clone

DCS-341, monoclonal

clone

DCS-31, monoclonal

clone

polyclonal

Gene Information

human ... CDK7(1022)

Gene Information

human ... CDC7(8317)

Gene Information

human ... CDK4(1019)
mouse ... Cdk4(12567)
rat ... Cdk4(94201)

Gene Information

human ... CDK6(1021)

species reactivity

human

species reactivity

human

species reactivity

rat, mouse, human

species reactivity

human

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

affinity isolated antibody

General description

Cell division protein kinase 7 is an enzymes encoded by the CDK7 gene in humans. The protein belongs to cyclin-dependent protein kinase (CDK) family. CAK is a CDK-cyclin complex implicated in the control of multiple cell cycle transitions. The structure of CDK7 facilitates the inhibitors with differential specificity for the different CDKs and can be used in different biological processes.

Specificity

The antibody reacts specifically with Cdk7 (40 kDa catalytic subunit of Cdk activating kinase).

Immunogen

recombinant human Cdk7 protein.

Application

Monoclonal Anti-Cdk7/CAK antibody produced in mouse is suitable for the following applications:
  • Immunocytochemistry
  • Immunohistochemistry (frozen sections)
  • Immunoprecipitation
  • Microarray
  • Western blotting at a dilution of 1:50,000 using a cultured human cell line extract

Biochem/physiol Actions

Cyclin-dependent kinase (CDK) Activating Kinase (CAK) is involved in activation and phosphorylation of CDK1, CDK2, CDK4 and CDK6 and regulation of the cell cycle. It is required for cell cycle progression and it could be a target for cancer therapy. CAK plays a central role in regulation of messenger RNA synthesis by either inhibition of RNA polymerase II-catalyzed transcription or stimulation of transcription through association with basal transcription repair factor IIH. Phosphorylation by the CDK-activating kinase (CAK) is required for the activation of cyclin-dependent kinases.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    067M4838V
    22170426
    037H4834

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    V Ossipow et al.
    Cell, 83(1), 137-146 (1995-10-06)
    The protein kinase MO15/CDK7 has recently been shown to be associated with the general transcription factor TFIIH and to be capable of phosphorylating the RNA polymerase II carboxy-terminal domain. Here, we show that a monoclonal MO15/CDK7 antibody coimmunoprecipitates, from a
    Stéphane Larochelle et al.
    Methods in molecular biology (Clifton, N.J.), 296, 279-290 (2004-12-04)
    All cyclin-dependent kinases (CDKs) involved in eukaryotic cell cycle control require phosphorylation at a conserved threonine (or serine) residue within the activation- or T-loop to attain full enzymatic activity. The enzyme responsible for this activating phosphorylation, the CDK-activating kinase (CAK)
    T P Mäkelä et al.
    Nature, 371(6494), 254-257 (1994-09-15)
    The eukaryotic cell cycle is regulated by the sequential activation of cyclin-dependent kinases (CDKs). CDK activation is dependent on cyclin binding and phosphorylation of a conserved threonine (T161 in Cdc2) mediated by the CDK-activating kinase CAK. A CDK-related kinase, MO15
    Graziano Lolli et al.
    Cell cycle (Georgetown, Tex.), 4(4), 572-577 (2005-05-07)
    The Cyclin-dependent kinase (CDK) Activating Kinase (CAK) is responsible for the activating phosphorylation of CDK1, CDK2, CDK4 and CDK6 and regulation of the cell cycle. The kinase is composed of three subunits: CDK7, Cyclin H and MAT1 (ménage a trois).
    R P Fisher et al.
    Cell, 78(4), 713-724 (1994-08-26)
    Phosphorylation by the CDK-activating kinase (CAK) is a required step in the activation of cyclin-dependent kinases. We have purified CAK from mammalian cells; the enzyme comprises two major polypeptides of 42 and 37 kDa. Protein sequencing indicates that the 42
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service