C7902

Calcium chloride dihydrate

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

• Empirical Formula (Hill Notation): CaCl₂ · 2H₂O
• CAS Number: 10035-04-8
• Molecular Weight: 147.01
• EC Number: 233-140-8
• MDL number: MFCD00149613
• UNSPSC Code: 12352302
• PubChem Substance ID: 24892994
• NACRES: NA.71
• assay: ≥99.0%
• form: powder
• solubility: H₂O: 294 mg/mL, clear, colorless

Properties

• product line: BioReagent
• Quality Level: 200
• assay: ≥99.0%
• form: powder
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable; cell culture | plant: suitable
• mp: 176 °C (dec.) (lit.)
• solubility: H₂O: 294 mg/mL, clear, colorless
• SMILES string: O.O.Cl[Ca]Cl
• InChI: 1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
• InChI key: LLSDKQJKOVVTOJ-UHFFFAOYSA-L

Description

General description

Calcium chloride (CaCl₂) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl₂ is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl₂-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl₂ is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaCl₂ along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaCl₂ on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.

Application

Calcium chloride dihydrate has been used:

• in the formulation of media
• in sperm collection and preparation
• in cell culture and protoplast preparation

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves