D218

Monoclonal Anti-Dihydropyridine Receptor (α₁ Subunit) antibody produced in mouse

clone 1A, buffered aqueous solution

• MDL number: MFCD01633297
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• conjugate: unconjugated
• Quality Level: 200
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 1A, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~200 kDa
• species reactivity: human (weakly), guinea pig, mouse, rat, rabbit
• technique(s): immunohistochemistry (frozen sections): 1:200; immunoprecipitation (IP): suitable; western blot (chemiluminescent): 1:500
• isotype: IgG1

Description

General description

DHP receptor is composed of at least four subunits. The α1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated Na+ channel. The α2 subunit is a large glycoprotein and the other 2 subunits are termed β and γ.

Specificity

Reacts specifically with the α₁ subunit of the DHP receptor. May be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

Immunogen

rabbit muscle transverse tubule dihydropyridine (DHP) receptors.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse is suitable for immunoprecipitation, immunohistochemistry at a working dilution of 1:500 and immunoblotting at 1:2000. It was used for the detection and localization of DHPR-1α by western blotting in skeletal muscle tissue sections of mice in a study. The antibody may be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells.

Biochem/physiol Actions

The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type Ca²⁺ channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)