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Key Documents

F6765

Sigma-Aldrich

Fetal Bovine Serum

USA origin, Charcoal Stripped, sterile-filtered, suitable for cell culture

Synonym(s):

Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.71
biological source:
bovine fetus
origin:
USA origin
sterility:
sterile-filtered
shipped in:
dry ice

biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Select

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin (typically ≤1 EU/ml)

shipped in

dry ice

storage temp.

−20°C

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Application

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture. Charcoal stripped FBS is often used instead of FCS to minimize the level of androgen and other hormones provided in 10% serum supplemented media.

Biochem/physiol Actions

Charcoal Stripped Fetal Bovine Serum (FBS) is available for researchers requiring decreased levels of various hormones in their studies. Dextran treated Charcoal is used to selectively remove hormones without nonspecific loss of other serum components. The effectiveness of hormone removal is assessed on the basis of reductions in the levels of T3, T4, and Progesterone pre and post treatment.

This product is designed for researchers involved in studying processes influenced by steroid hormones such as estrogen stimulation and the obesity process and other areas that benefit from reduced hormone levels such as certain types of viral infections.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Other Notes

The product is not available in Australia. Please view our protocol for Charcoal-stripping FBS to deplete hormones here.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves


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