H3884

Hyaluronidase from bovine testes

Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid

• Synonym(s): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• CAS Number: 37326-33-3
• EC Number: 3.2.1.35 (BRENDA, IUBMB)
• EC Number: 253-464-3
• MDL number: MFCD00131351
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bovine testis
• Quality Level: 200
• conjugate: conjugate (Glucosaminoglycan)
• type: Type IV-S
• form: lyophilized powder (essentially salt-free)
• specific activity: 750-3000 units/mg solid
• mol wt: ~55 kDa (four subunits of 14 kDa each)
• UniProt accession no.: Q5E985; Q8SQG8
• application(s): diagnostic assay manufacturing
• relevant disease(s): cancer
• storage temp.: −20°C
• Gene Information: cow ... HYAL1(515397), HYAL2(281838)

Description

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.

These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)