Product Name
Keratinocyte Growth Factor human, KGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
biological source
human
recombinant
expressed in HEK 293 cells
assay
>95% (SDS-PAGE)
form
lyophilized powder
potency
4-20 ng/mL EC50
quality
endotoxin tested
mol wt
dimer 17-30 kDa (glycosylated)
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... FGF7(2252)
Looking for similar products? Visit Product Comparison Guide
General description
Keratinocyte Growth Factor (KGF) belongs to the fibroblast growth factor 7 (FGF-7) family. It is produced by the mesenchymal cells, γδ T cells, and fibroblasts in several organs. The KGF gene is located on the human chromosome at 15q21.2.
Application
Keratinocyte Growth Factor (KGF) human has been used as a component of the keratinocyte differentiation medium.
Biochem/physiol Actions
Keratinocyte Growth Factor (KGF) exerts paracrine effects on mesenchymal cells. It exhibits cryoprotective effects on epithelial cells of the lungs, gastrointestinal tract, and the lungs. KGF participates in the activation of IIIb isoform of the fibroblast growth factor (FGF)-receptor 2 (FGFR2-IIIb) present on keratinocytes. This activation further favors proliferation, migration, and survival of keratinocytes.
Physical form
Lyophilized from a 0.2 μm filtered solution of 1x PBS.
Preparation Note
HumanKine Keratinocyte Growth Factor (KGF) is expressed as glycosylated 17 and 30 kDa monomers in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Analysis Note
The activity was determined by the dose-dependent stimulation of the proliferation of 4MBr-5 cells (monkey epithelial cell line).
Legal Information
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service