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Sigma-Aldrich

Anti-Human IgG (whole molecule) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-IgG antibody, Rabbit anti-Human IgG

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46
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biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:80,000
quantitative precipitin assay: 3.0-4.0 mg/mL

shipped in

dry ice

storage temp.

−20°C

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This Item
716944739448778095
form

dispersion (H2O)

form

dispersion (H2O), nanowires

form

liquid (suspension), nanowires

form

liquid (suspension), nanowires

contains

<3.5% CTAB as stabilizer

contains

CTAB as stabilizer

contains

-

contains

-

concentration

>50 μg/mL

concentration

≥50 μg/mL

concentration

0.5% (isopropyl alcohol suspension)

concentration

0.5% (isopropyl alcohol suspension)

diam. × L

30 nm × 6,000 nm

diam. × L

30 nm × 4,500 nm

diam. × L

100 nm × 6 μm

diam. × L

200  nm × 10 μm

pH

3.0-5.0

pH

3.0-5.0

pH

-

pH

-

density

1 g/mL at 25 °C

density

1.00 g/mL at 25 °C

density

-

density

0.779 g/mL at 25 °C

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulin have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein, usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.

Specificity

The antiserum is determined to be immunospecific for human IgG. Reactivity with light chains is observed.

Application

Anti-Human IgG (whole molecule) antibody produced in rabbit has been used in immunoprecipitation and enzyme linked immuno sorbent assay (ELISA).
Anti-Human IgG (whole molecule) antibody produced in rabbit was coated on Sepharose beads to immunoprecipitate IgG from HUVEC cultured conditioned medium.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Storage and Stability

For continuous use, store at 2-8 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is notrecommended.If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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