L3790

Lysozyme from chicken egg white

10 mg/mL

• Synonym(s): Mucopeptide N-acetylmuramoylhydrolase, Muramidase
• CAS Number: 12650-88-3
• EC Number: 3.2.1.17 (BRENDA, IUBMB)
• MDL number: MFCD00131557
• UNSPSC Code: 12352204
• NACRES: NA.56

Properties

• biological source: chicken egg white
• Quality Level: 200
• form: liquid
• mol wt: single-chain 14.3 kDa
• concentration: ≥400000 units/mL; 10 mg/mL
• technique(s): cell based assay: suitable
• suitability: suitable for cell lysis
• UniProt accession no.: P00698
• application(s): cell analysis
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: chicken ... LYZ(396218)

Description

General description

Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction.

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Suitable for the purification of both DNA and protein from bacteria.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.

Suitability

Tested for suitability with CelLytic^™ B in protein extraction from bacteria.

Unit Definition

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Physical form

Ready to use solution:10 mg/mL lysozyme in 25 mM sodium acetate pH 4.5 with 50% glycerol.

Solution in 50% glycerol, 25 mM Sodium Acetate

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)