biological source
chicken liver
Quality Level
form
ammonium sulfate suspension
specific activity
10-30 units/mg protein (modified Warburg-Christian)
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
General description
Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.
Application
Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.
Biochem/physiol Actions
Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.
Unit Definition
One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO2, and NADPH per min at pH 7.4 at 25 °C.
Physical form
Suspension in 2.9 M (NH4)2SO4 solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na4EDTA, pH 6.0
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service