MP0035

LookOut^® Mycoplasma PCR Detection Kit

Optimized for use with JumpStart^™ Taq DNA Polymerase, D9307.

• Synonym(s): PCR-based mycoplasma screening
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• quality: Not suitable for clinical diagnostic use. Will not detect clinically relevant species such as M. pneumoniae and U. urealyticum
• Quality Level: 100
• packaging: pkg of 1 kit
• technique(s): PCR: suitable
• application(s): detection; microbiology
• compatibility: Optimized for use with JumpStart^™ Taq DNA Polymerase, D9307.
• storage temp.: 2-8°C

Description

General description

The LookOut^® Mycoplasma PCR Detection Kit utilizes the polymerase chain reaction (PCR), which is established as the method of choice for highest sensitivity in the detection of Mycoplasma, Acholeplasma, and Ureaplasma contamination in cell cultures and other cell culture derived biologicals. Kit has been successfully tested for the ability to amplify Mycoplasma fermentans genomic DNA in concentrations of 20,000 to 20 copies.
The reaction tubes included with the kit are pre-coated with appropriate dNTPs, primers, and loading dye. Total assay time is greatly reduced compared to general protocols that require individual loading of reaction tubes.

Application

Mycoplasma PCR Detection Kit Video Protocol

The LookOut^® Mycoplasma PCR Detection Kit has been used for screening of mycoplasma infection.[1][2]

Other Notes

The kit has been optimized for use with Product No. D9307. Any DNA polymerase with a 3’-5’ exonuclease activity is NOT recommended. These include Tli, Pfu,and Pwo. Those DNA polymerases that may be used include Taq, Tfl, and Tth.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable