SAB4702003

Monoclonal Anti-CD123 PE produced in mouse

• Synonym(s): IL3RA, MGC34174, hIL-3Ra
• UNSPSC Code: 12352203

Properties

• conjugate: phycoerythrin (R-PE) conjugate
• Quality Level: 100
• antibody product type: primary antibodies
• clone: 6H6, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: suitable
• isotype: IgG1
• UniProt accession no.: P26951
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... IL3RA(3563)

Description

General description

CD123 is the alpha chain of interleukin 3 receptor (IL-3R alpha). This subunit heterodimerizes with the interleukin 3 receptor beta chain (CD131), which is shared with other receptors. CD123 interacts with IL-3 specifically, but with low affinity, and association with the beta subunit confers high affinity binding to the receptor heterodimer. Both chains are required for signaling, but receptor activation and signal transduction depend on IL-3 binding to CD123 as the initial step.

Specificity

The mouse monoclonal antibody 6H6 recognizes an extracellular epitope of CD123 (interleukin 3 receptor alpha), a 60-70 kDa transmembrane protein expressed by myeloid precursors, megakaryocytes, macrophages, dendritic cells, mast cells, basophils, and some B cells. This antibody does not inhibit IL-3 binding to its receptor.

Immunogen

IL3 receptor alpha chain expressed on the surface of transiently transfected COS cells

Physical form

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Storage and Stability

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable