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SRP0373

Sigma-Aldrich

CDK4/Cyclin D1 active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)

Synonym(s):

BCL1, CCND1, Cell division protein kinase 4, Cyclin-dependent kinase 4, PRAD1, PSK-J3

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

29 kDa (Cyclin D1)
 34 kDa (CDK4)

packaging

pkg of 20 μg

solubility

water: soluble

NCBI accession no.

NM_000075 (CDK4)
NM_053056 (cyclin D1)

application(s)

life science and biopharma

shipped in

dry ice

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C0620SRP5009SRP5012
CDK4/Cyclin D1 active human recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)

SRP0373

CDK4/Cyclin D1 active human

CDK4/Cyclin D1, active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

C0620

CDK4/Cyclin D1, active, GST tagged human

CDK1/CyclinB1, active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

SRP5009

CDK1/CyclinB1, active, GST tagged human

CDK9/CyclinK, active, GST tagged human PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

SRP5012

CDK9/CyclinK, active, GST tagged human

biological source

human

biological source

-

biological source

-

biological source

-

Gene Information

human ... CCND1(595), CDK4(1019)

Gene Information

human ... CCND1(595), CDK4(1019)

Gene Information

human ... CCNB1(891), CDK1(983)

Gene Information

human ... CCNK(8812), CDK9(1025)

assay

≥90% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

application(s)

life science and biopharma

application(s)

-

application(s)

-

application(s)

-

form

aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

General description

Research area: Apoptosis

Cyclin D1 (CCND1) is a proto-oncogene. It is one of the three D-type cyclins. The gene encoding it is localized on human chromosome 11q13.[1][2][3] Cyclin dependent kinase-like 4 (CDKL4) is part of the cyclin-dependent protein kinase (CDK) family.[4] The gene encoding it is localized on human chromosome 12q14-15.[5]
Recombinant, full length human CDK4 (Genbank Accession No. NM_000075), amino acids 16-271, with C-terminal His-tag and modifications (amino acids 43G-47G were replaced with EE, and T172A), MW=34 kDa, and human Cyclin D1 (Genbank accession number NM_053056), MW 29 kDa, were co-expressed in a Baculovirus-infected Sf9 cells expression system.

Biochem/physiol Actions

Cyclin D1 (CCND1) regulates the Wnt signaling pathway. It also regulates the progression of the cell cycle from the G1 to S phase in many cell types. CCND1 phosphorylates and inactivates retinoblastoma (Rb) protein. Cyclin D1 has been associated with mantle cell lymphoma. This protein is overexpressed in multiple cancers like hepatocellular carcinoma.[1][2][3] Cyclin dependent kinase-like 4 (CDKL4) has been associated with various tumors and colorectal cancer.[4] The protein regulates apoptosis of nucleated cells by modifying transcriptional regulation of genes governing cell proliferation and cell death.[6] Like CCND1, it also modulates the progression of cycle from G1 to S phase.[7]

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Lot/Batch Number
4009420312

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