technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 5 cm x 3.0 mm I.D., 2.7 μm particles (53811-U)
mobile phase: [A] 20 mM potassium phosphate, dibasic, pH 7; [B] methanol
gradient: 0.5% B for 1.3 min, 0.5-30%B over 1.7 min; to 30% B in 1.4 min, 2 min equilibration at 0.5% B
flow rate: 1.0 mL/min
pressure: 4130 psi (285 bar)
column temp.: 35 °C
detector: UV, 210 nm
sample: 9 vitamins in water, 10 μg/mL for nicotinic acid and folic acid, 12 μg/mL for thiamine hydrochloride and cyanocobalamin, (20 μg/mL for pyridoxine, 30 μg/mL for riboflavin, 40 μg/mL for niacineamide, 100 μg/mL for calcium pantothenate, 120 μg/mL for biotin)

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products