Skip to Content

Apoptosis-inducing effects of Melissa officinalis L. essential oil in glioblastoma multiforme cells.

Apoptosis-inducing effects of Melissa officinalis L. essential oil in glioblastoma multiforme cells.

Cancer investigation (2014-04-22)

Rafaela Muniz de Queiroz, Christina Maeda Takiya, Lívia Paes Tavares Pacheco Guimarães, Gleice da Graça Rocha, Daniela Sales Alviano, Arie Fitzgerald Blank, Celuta Sales Alviano, Cerli Rocha Gattass

ABSTRACT

Current therapies for glioblastoma multiforme (GBM) are not effective. This study investigated the activity of the M. officinalis essential oil (EO) and its major component (citral) in GBM cell lines. Both EO and citral decreased the viability and induced apoptosis of GBM cells as demonstrated by DNA fragmentation and caspase-3 activation. Antioxidant prevented citral-induced death, indicating its dependence on the production of reactive oxygen species. Citral downmodulated the activity and inhibited the expression of multidrug resistance associated protein 1 (MRP1). These results show that EO, through its major component, citral, may be of potential interest for the treatment of GBM.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

N-Acetyl-L-cysteine, BioXtra, ≥99% (TLC)

Sigma-Aldrich

Dimethyl sulfoxide, JIS special grade, ≥99.0%

Sigma-Aldrich

Dimethyl sulfoxide, SAJ first grade, ≥99.0%

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.0%, suitable for absorption spectrum analysis

Sigma-Aldrich

Dimethyl sulfoxide, suitable for HPLC

Sigma-Aldrich

Dimethyl sulfoxide solution, 50 wt. % in H₂O

Sigma-Aldrich

N-Acetyl-L-cysteine, SAJ special grade, 98.0-102.0%

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.5%

Sigma-Aldrich

8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)

Sigma-Aldrich

N-Acetyl-L-cysteine, Sigma Grade, ≥99% (TLC), powder

Supelco

Citral, analytical standard

Supelco

N-Acetyl-L-cysteine, Pharmaceutical Secondary Standard; Certified Reference Material

Fluorescein, European Pharmacopoeia (EP) Reference Standard

Sigma-Aldrich

Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water

USP

Dimethyl sulfoxide, United States Pharmacopeia (USP) Reference Standard

Sigma-Aldrich

Dimethyl sulfoxide, Vetec^™, reagent grade, 99%

Dimethyl sulfoxide, European Pharmacopoeia (EP) Reference Standard

Sigma-Aldrich

Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)

Supelco

Dimethyl sulfoxide, for inorganic trace analysis, ≥99.99995% (metals basis)

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture

Sigma-Aldrich

Propidium iodide solution

Sigma-Aldrich

Citral, 95%

Sigma-Aldrich

Rhodamine 123, mitochondrial specific fluorescent dye

Sigma-Aldrich

Rhodamine 123, BioReagent, for fluorescence, ≥85% (HPLC)

Sigma-Aldrich

DAPI, for nucleic acid staining

Sigma-Aldrich

Dimethyl sulfoxide, for molecular biology

Sigma-Aldrich

Dimethyl sulfoxide, anhydrous, ≥99.9%

Sigma-Aldrich

Citral, natural, ≥96%, FCC, FG

Supelco

Dimethyl sulfoxide, analytical standard

Sigma-Aldrich

N-Acetyl-L-cysteine, BioReagent, suitable for cell culture