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Test Author Name, MBBS, MD, Ph.D1,2, Test Author Name 2, Test Author Title 21, Test Author Name 3, Test Author Title 32

Westorn Bloting Princliple and Methods, Chapter 1

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Left Primary Content

  • Mammalian Cell Culture
    Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
  • Microbial Cell Culture
    An overview of microbiological cell culture and its applications in molecular biology, cloning, and recombinant protein expression as well as cultivation for the isolation, differentiation, and identification of disease-causing microbes in clinical researc
  • See All Link Left (0)

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    Plant cell and tissue culture in commercial plant production, conservation, and plant research. Meristem culture, virus-free plant propagation, protoplast and haploid plant production.
  • Primary Cell Culture Techniques
    Cultivate mammalian primary cells for ADME/Tox screening, maintaining physiological relevance for accurate results.
  • Stem Cell Culture
    An overview of different stem cell types, stem cell culture basics and stem cell applications in basic research and clinical research applications, such as tissue regeneration, genetic diseases and cancer.
  • Cell Culture Media Preparation
    Culture media preparation for in vitro cell cultivation including preparation of different media formats, sterilization methods, cell growth and regulation supplements, media storage and use.
  • Cell Growth & Maintenance
    A synopsis of the conditions, media, nutrients, cultureware, and environments required for successful propagation of mammalian and yeast cells. Nutrient reagents, sterile technique and filtration, and requirements for 2D, 3D, and suspension cultures are described.
  • Transfection & Gene Editing
    Cell transfection principles, common transfection methods, and the role of transfection in gene editing and silencing for research, drug discovery, recombinant protein production.
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Reference Headline

1.
Given Family Name TGN. 2019. Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy. 3rd Edition. Bangalore: Elsevier.
2.
2019. Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy. 3rd Edition. NY: Elsevier.
3.
Given Family Name 2 GN2. 2019. Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy. 4th Edition. NJ: Elsevier.
4.
Given Family Name TGN. 2019. Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy. 4th Edition. Bangalore: Elsevier.
5.
Family Name 2 GN2. 2019. Protein Kinase Inhibitors as Sensitizing Agents for Chemotherapy. 5th Edition. NY: Elsevier.
6.
Family Name GN. 2020. Webpage Title. [Internet]. Bangalore: Bangalore Publication.[updated 28 Dec 2020; cited 28 Dec 2020]. Available from: https://www.google.com

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Atomic spectroscopy uses the energy absorbed or emitted by electrons to identify and quantify the elemental composition of a sample. It includes various analytical techniques, such as AAS, AES, FAA, GFAA, ICP-OES, ICP-MS and XRF.
Calibration, Qualification & Validation
Calibration, Qualification & Validation
Reference materials selection is vital for accurate testing, supporting method validation, calibration, and measurement uncertainty assessment.
Gas Chromatography (GC)
Gas Chromatography (GC)
Gas chromatography separates volatile compounds in the gas phase, applied in various industries for quality control.
Large Molecule HPLC
Large Molecule HPLC
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
Liquid Chromatography at Low Pressures
Liquid Chromatography at Low Pressures
Low pressure liquid chromatography (LPLC) is a chromatographic technique that operates at low pressures to drive the mobile phase onto the column containing a stationary phase by the action of a pump.
Small Molecule HPLC
Small Molecule HPLC
Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).
Related Content Grid Headline
Overridden Page Title
Mammalian Cell Culture
Applications, principles, and methods for in vitro mammalian cell culture including links to articles, protocols, and videos for proper aseptic technique, passaging and subculturing, and culture media considerations.
Microbial Cell Culture
An overview of microbiological cell culture and its applications in molecular biology, cloning, and recombinant protein expression as well as cultivation for the isolation, differentiation, and identification of disease-causing microbes in clinical researc
Plant Tissue Culture
Plant cell and tissue culture in commercial plant production, conservation, and plant research. Meristem culture, virus-free plant propagation, protoplast and haploid plant production.
Primary Cell Culture Techniques
Cultivate mammalian primary cells for ADME/Tox screening, maintaining physiological relevance for accurate results.
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