Transfection Reagent Selection Guide
Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a variety of cells (as indicated in the product manual). Scientific publications continue to add to the growing list of cells that are transfected well with our transfection reagents. You can view these publications from the links on the product pages. Use the chart below to determine which reagent is right for your cells. For more help on choosing a reagent, review our Introduction to Transfection.
See the Roche X-tremeGENE™ Transfection Database for hundreds more protocols and articles.
✓✓ = excellent ✓ = good/compatible
| Reagent | Escort™ III Transfection Reagent (L3037) | Escort™ IV Transfection Reagent (L3287) | Calcium Phosphate Transfection Kit (CAPHOS) | DOTAP Liposomal Transfection Reagent (11202375001) | NeuroPorter™ Transfection Kit (NPT01) |
|---|---|---|---|---|---|
| General Guidelines | Primary cells, sensitive cells | Insect cells, mammalian cell lines, some primary cells | Common cell lines | Common cell lines | Neuronal cell lines, glial cells, some primary neurons |
| Mammalian Cell Lines | |||||
| 16HBE14o- | |||||
| 3LL | |||||
| 6CSFMEo | |||||
| A549 | ✓ | ✓ | |||
| B16-F10 | |||||
| BAEC | ✓ | ||||
| BHK-21 | ✓ | ||||
| C2C12 | ✓ | ✓ | |||
| Ca Ski | |||||
| CHO (image) | ✓✓ | ✓ | |||
| CHOK1 | ✓✓ | ✓ | ✓ | ||
| COS (image) | ✓ | ||||
| COS-1 | ✓ | ✓ | |||
| COS-7 | ✓ | ✓ | ✓ | ||
| CV-1 | |||||
| D 407 | |||||
| DHD Pro.b | |||||
| H9c2 | ✓ | ||||
| HaCaT | ✓ | ||||
| HEK293 | ✓✓ | ✓ | ✓ | ||
| HeLa | ✓ | ✓✓ | ✓ | ✓ | |
| HepG2 | ✓ | ✓ | |||
| IMR90 | ✓ | ||||
| Jurkat | ✓✓ | ||||
| L929 | |||||
| LNCaP | ✓ | ||||
| MCF-7 | ✓ | ✓ | |||
| MDA-MB-435 | ✓ | ||||
| MDCK | ✓ | ||||
| Neuro2A | |||||
| NIH3T3 | ✓ | ✓✓ | ✓ | ✓ | |
| PC-12 (image) | ✓✓ | ✓ | |||
| SAOS-2 | ✓ | ||||
| SHEP | |||||
| SH-SY5Y | ✓ | ||||
| SiHa | |||||
| SKOV3 | |||||
| T-24 | ✓ | ||||
| Vero | ✓ | ✓ | |||
| WI-38 | ✓ | ✓ | |||
| Insect Cell Lines | |||||
| Sf9 (adherent) | ✓✓ | ✓ | |||
| Sf9 (suspension) | ✓✓ | ||||
| S2 (D. mel.) (image) | ✓✓ | ||||
| S2R+ | ✓✓ | ||||
| T. Ni. | ✓ | ✓✓ | |||
| Primary Cells | |||||
| Aortic smooth muscle cells (rat) | ✓ | ||||
| Blastocyst (mouse) | ✓ | ||||
| Cardiac myocytes (human) | ✓ | ||||
| Cardiomyocytes (rat neonatal) | ✓✓ | ||||
| Cardiomyocytes (rat) | ✓✓ | ||||
| Fibroblasts ( human skin) | |||||
| Fibroblasts (rat) | ✓✓ | ||||
| Germ cells (male rat) | ✓✓ | ||||
| Hepatocytes (hampster) | ✓✓ | ||||
| Hepatocytes (rat) | ✓✓ | ||||
| HLMVEC | |||||
| H-MVEC | |||||
| HUVEC | ✓ | ||||
| Keratinocytes (human) (image) | ✓✓ | ✓ | |||
| Myoblasts (rat) | ✓ | ||||
| Myoblasts (quail) (image) | ✓✓ | ✓ | |||
| Oviduct cells (bovine) | |||||
| Pre-adipocytes (human) | |||||
| Retinal neurons (chick) | ✓✓ | ||||
| Tracheobronchial cells (human) | ✓✓ | ||||
| Neuronal & Glial Cells | |||||
| Astrocytes (image) | ✓ | ||||
| C6 Glioma | ✓ | ||||
| Hippocampal neurons (primary rat) | ✓ | ||||
| NT2 | ✓ | ||||
| NT2 (differentiated neurons) | ✓ | ||||
| Reagent | GeneJuice® Transfection Reagent (70967) | 293-Free™ Transfection Reagent (72181) | NovaCHOice® Transfection Kit (72622-M) | RiboJuice™ siRNA Transfection Reagent (71115) | Insect GeneJuice® Transfection Reagent (71259) | Polybrene Infection / Transfection Reagent (TR-1003) |
|---|---|---|---|---|---|---|
| General guidelines | Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammallian cells. | Animal-free polycationic liposomal transfection reagent optimized for the transfection of HEK293 cells grown in suspension culture. | Optimized transfection reagent specifically developed for mammalian protein production in chinese hamster ovary (CHO) cells. | Optimized, easy-to-use siRNA transfection reagent formulated for minimal toxicity. | A proprietary liposome formulation optimized for maximal transfection efficiency of Sf9 insect cells. | A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors. |
| L428 | ✓✓ | |||||
| L591 | ✓✓ | |||||
| Neuro-2A | ✓✓ | |||||
| 10T1/2 | ||||||
| 293T | ✓✓ | ✓✓ | ||||
| 3T3 NIH | ✓✓ | ✓✓ | ||||
| 3T3 Swiss | ||||||
| 3T3-L1 | ||||||
| A204 | ||||||
| A431 | ||||||
| A549 | ✓✓ | |||||
| alpha TC1-6 | ||||||
| AR 42J | ||||||
| As4.1 | ||||||
| AtT-20 | ||||||
| B50 | ||||||
| BC-1 | ||||||
| BC-2 | ||||||
| BC3 | ||||||
| BCBL | ||||||
| BHK-21 | ||||||
| C3H/10T1/2 | ||||||
| C6 | ||||||
| C2C12 | ||||||
| Caki-1 | ||||||
| Calpan-1 | ||||||
| Calu-1 | ||||||
| Calu-6 | ||||||
| CCL-131 | ||||||
| CFPAC-1 | ||||||
| Chang Liver | ||||||
| CHO | ✓✓ | ✓✓ | ✓✓ | |||
| CHO-7 | ✓✓ | ✓✓ | ✓✓ | |||
| CHO-IR | ✓✓ | ✓✓ | ✓✓ | |||
| CHO-K1 | ✓✓ | ✓✓ | ✓✓ | |||
| COS-1 | ||||||
| COS-7 | ✓✓ | |||||
| CS-1 | ||||||
| CV-1 | ||||||
| Daudi | ||||||
| DDTI MF-2 | ||||||
| DT40 | ||||||
| ECV304 | ||||||
| EL4 | ||||||
| EVSCC17M | ||||||
| H9c2 | ||||||
| HCT-116 | ||||||
| HEK293 | ||||||
| HeLa | ✓✓ | |||||
| HeLa B | ✓✓ | |||||
| HeLa T4 | ✓✓ | |||||
| Hep 3B2.1-7 | ||||||
| HepG2 | ✓✓ | |||||
| Hepa 1-6 | ||||||
| Ht-29 | ||||||
| HTB-37 | ||||||
| HTB-45 | ||||||
| Huh-7 | ||||||
| HUVEC | ||||||
| IC21 | ||||||
| IEC-6 | ||||||
| JEG-3 | ||||||
| Jurkat | ||||||
| KB | ||||||
| L-6 | ||||||
| L-929 | ||||||
| MA-10 | ||||||
| McA-RH7777 | ||||||
| MCF-7 | ✓✓ | |||||
| MCF-10-2A | ||||||
| MDCK | ||||||
| Melanocyte | ||||||
| MG-63 | ||||||
| Neuro 2A | ||||||
| Neuroblastoma | ||||||
| NRK | ||||||
| NT2/D1 | ||||||
| OV-1063 | ||||||
| OVCAR3 | ||||||
| P4 | ||||||
| P19 | ||||||
| PC12 | ||||||
| PA317 | ||||||
| PAM212 | ||||||
| R2C | ||||||
| RAW 264.7 | ||||||
| RBL-2H3 | ||||||
| RMP-41 | ||||||
| SAOS-2 | ||||||
| SC-1 | ||||||
| Schneider line2 | ||||||
| SK-N-MC | ||||||
| SK-N-SH | ||||||
| SKOV3 | ||||||
| STO | ||||||
| SW-480 | ||||||
| SW-837 | ||||||
| T3M4 | ||||||
| TM4 | ||||||
| U937 | ||||||
| UCD | ||||||
| Vero | ||||||
| Sf9 (adherent) | ✓✓ | |||||
| Sf9 (suspension) | ✓✓ | |||||
| S2 (D. mel.) | ||||||
| S2R+ | ||||||
| T. Ni. | ||||||
| rat HSC | ✓✓ | |||||
| Chicken embryo fibroblases | ✓✓ | |||||
| Myeloid cells | ✓✓ | |||||
| Aortic smooth muscle cells | ✓✓ | |||||
| Astrocytes | ||||||
| Angioblasts | ||||||
| Chondrocytes | ||||||
| Chromaffin cells | ||||||
| Epithelial cells: | ||||||
| mammary | ||||||
| prostate | ||||||
| tracheal | ||||||
| Fibroblasts | ||||||
| Keratinocytes | ✓✓ | |||||
Find more protocols and selection guides in the Molecular Biology Guide.
Materials
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