T2819

Trizma^® hydrochloride solution

pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture

• Synonym(s): Tris hydrochloride solution
• UNSPSC Code: 12161700
• NACRES: NA.25

Properties

• grade: BioPerformance Certified; Molecular Biology
• Quality Level: 200
• form: solution
• concentration: 1 M
• technique(s): cell culture | mammalian: suitable
• impurities: DNase, RNase, Protease, none detected; bioburden, tested; endotoxin, tested; ≤5 ppm Heavy metals (as Pb)
• pH: 9.0
• useful pH range: 7.0-9.0
• absorption: ≤0.05 at 290 at 40%

Description

General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Tris hydrochloride is used to prepare buffer, mainly at a physiological range of 7.3 to 7.5. This buffer is well-suited with biological fluids. It is used in laboratories as primary pH standard in clinical laboratories.

Application

Trizma^® hydrochloride solution has been used:

• to neutralize IgG bound to protein A/G column
• as a component in BigDye buffer for sequencing reaction
• to block slides

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany