A8200

Aminopeptidase from Aeromonas proteolytica

lyophilized powder, 50-150 units/mg protein

• Sinónimos: AAP, Aminopeptidase from Vibrio proteolyticus, bacterial leucyl aminopeptidase
• CAS Number: 37288-67-8
• Número CE: 3.4.11.10 (BRENDA, IUBMB)
• Número CE: 232-874-6
• Número MDL: MFCD00166325
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• grado: Proteomics Grade
• Nivel de calidad: 300
• Formulario: lyophilized powder
• actividad específica: 50-150 units/mg protein
• mol peso: 29.5 kDa
• composición: Protein, ~40% biuret
• solubilidad: H₂O: soluble 0.9-1.1 mg/mL, clear, colorless
• actividad extraña: endopeptidase, essentially free
• temp. de almacenamiento: −20°C

Descripción

Descripción general

A zinc-containing enzyme.

Especificidad

Catalyzes the release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Aplicación

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.

Acciones bioquímicas o fisiológicas

Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn²⁺ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase.

Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.

Definición de unidad

One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.

Forma física

Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Nota de preparación

Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution.

Información de seguridad

• Pictogramas: GHS08,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H315,H319,H334,H335
• Consejos de prudencia: P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)