C9584

Carboxypeptidase B from porcine pancreas

lyophilized powder

• Sinónimos: Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase
• CAS Number: 9025-24-5
• Número CE: 3.4.17.2 (BRENDA, IUBMB)
• Número CE: 232-788-9
• Número MDL: MFCD00081476
• Código UNSPSC: 12352204
• ID de la sustancia en PubChem: 24893175
• NACRES: NA.54

Propiedades

• origen biológico: Porcine pancreas
• Nivel de calidad: 200
• grado: Proteomics Grade
• Formulario: lyophilized powder
• actividad específica: ≥125 units/mg protein
• mol peso: 34,000 Da± 600
• purificado por: affinity chromatography
• composición: Protein, 40-70%
• actividad extraña: carboxypeptidase A ≤1%; chymotrypsin ≤0.1%; trypsin ≤5%
• temp. de almacenamiento: −20°C
• cadena SMILES: S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
• InChI: 1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
• Clave InChI: TWURVFFNODFJBJ-UHFFFAOYSA-N

Descripción

Aplicación

Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.

The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.

Acciones bioquímicas o fisiológicas

Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.

Envase

Package size based on protein content

Definición de unidad

One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.

Forma física

Contains HEPES buffer salts, zinc chloride and carbohydrate

Nota de preparación

Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

Nota de análisis

Protein determined by biuret.

Información de seguridad

• Pictogramas: GHS08,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H315,H319,H334,H335
• Consejos de prudencia: P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves