I0408

Invertase Glycoprotein Standard

BioReagent, from Saccharomyces cerevisiae

• Sinónimos: Invertase from baker’s yeast (S. cerevisiae), β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase
• CAS Number: 9001-57-4
• Número CE: 3.2.1.26 (BRENDA, IUBMB)
• Número CE: 232-615-7
• Número MDL: MFCD00131410
• Código UNSPSC: 12352202
• NACRES: NA.32

Propiedades

• origen biológico: Saccharomyces cerevisiae
• Nivel de calidad: 200
• Línea del producto: BioReagent
• Formulario: lyophilized powder
• mol peso: 60 kDa
• concentración: ≥0.5 mg/vial protein (E1%/280)
• temp. de almacenamiento: 2-8°C

Descripción

Aplicación

Invertase Glycoprotein Standard has been used:

• to generate N-linked glycan library
• as a negative control to study the binding of lectins to high mannose structures
• for sample pre-treatment in proteomic analyses to study drug-induced toxic epidermal necrolysis

The Invertase Glycoprotein Standard can be used to demonstrate N-glycosylation using PNGase F with both in-solution and in-gel procedures. The extent of deglycosylation can be assessed by mobility shift on SDS-PAGE gels.

Used in the production of confectionary foods and artificial honey.

Acciones bioquímicas o fisiológicas

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Otras notas

Invertase is an enzyme that catalyses the hydrolysis of sucrose into fructose and glucose. Invertase Glycoprotein Standard is the periplasmic (glycosylated form, external invertase) with 50% of its mass as polymannan. Since yeast can provide an alternative system for protein glycosylation that is similar to mammalian systems, periplasmic invertase is often used as a model for the study of the function of oligosaccharides in glycoproteins and for studies on glycoprotein biosynthesis.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)