NUC101

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)

• Sinónimos: Rapid mammalian nuclei isolation kit
• Código UNSPSC: 12352207
• NACRES: NA.32

Propiedades

• uso: sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)
• Nivel de calidad: 200
• caducidad: 1 yr at 2‑8 °C
• envase: pkg of 1 kit
• aplicaciones: cell analysis
• actividad extraña: nuclease and protease, free
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

Aplicación

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

Acciones bioquímicas o fisiológicas

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Otras notas

All procedures should be carried out on ice or 2-8°C.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable