SHC003V

MISSION^® pLKO.1-puro-CMV-TurboGFP^™ Positive Control Transduction Particles

Green fluorescent protein marker to monitor transduction efficiency

• Sinónimos: MISSION^®, MISSION^® TurboGFP^™ Control Transduction Particles
• Código UNSPSC: 41106609
• NACRES: NA.51

Propiedades

• Nivel de calidad: 100
• Línea del producto: MISSION^®
• concentración: ≥1x10⁶ VP/ml (via p24 assay)
• técnicas: capture ELISA: 10⁶ TU/mL using p24
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP^™ is an improved variant of the green fluorescent protein copGFP cloned from the copepode Pontellina plumata. The TurboGFP transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP (SHC003). It is a positive control to monitor transduction efficiency.

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 10⁶ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION^® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Aplicación

MISSION^® pLKO.1-puro-CMV-TurboGFP^™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.

Información legal

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TurboGFP is a trademark of Evrogen Co.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)