A8200

Aminopeptidase from Aeromonas proteolytica

lyophilized powder, 50-150 units/mg protein

• Sinônimo(s): AAP, Aminopeptidase from Vibrio proteolyticus, bacterial leucyl aminopeptidase
• CAS Number: 37288-67-8
• Número CE: 3.4.11.10 (BRENDA, IUBMB)
• Número CE: 232-874-6
• Número MDL: MFCD00166325
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• grau: Proteomics Grade
• Nível de qualidade: 300
• Formulário: lyophilized powder
• atividade específica: 50-150 units/mg protein
• peso molecular: 29.5 kDa
• composição: Protein, ~40% biuret
• solubilidade: H₂O: soluble 0.9-1.1 mg/mL, clear, colorless
• atividade externa: endopeptidase, essentially free
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

A zinc-containing enzyme.

Especificidade

Catalyzes the release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Aplicação

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.

Ações bioquímicas/fisiológicas

Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn²⁺ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase.

Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.

Definição da unidade

One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.

forma física

Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Nota de preparo

Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution.

Informações de segurança

• Pictogramas: GHS08,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H315,H319,H334,H335
• Declarações de precaução: P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, type N95 (US)