C3142

α-Chymotrypsin from bovine pancreas

(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

• Sinônimo(s): TLCK-Chymotrypsin
• CAS Number: 9004-07-3
• Número CE: 3.4.21.1 (BRENDA, IUBMB)
• Número CE: 232-671-2
• Número MDL: MFCD00130481
• Código UNSPSC: 12352204
• eCl@ss: 42010112
• NACRES: NA.54

Propriedades

• Nível de qualidade: 200
• tipo: Type VII
• Formulário: essentially salt-free, lyophilized powder
• atividade específica: ≥40 units/mg protein
• peso molecular: 25 kDa
• solubilidade: 1 mM HCl: soluble 10 mg/mL, clear
• nº de adesão UniProt: P00767
• temperatura de armazenamento: −20°C
• Informações sobre genes: cow ... CTRB1(618826)

Descrição

Aplicação

α-Chymotrypsin from Sigma has been used to determine the crystal structures of two homologous inhibitors (pars intercerebralis major peptide-C and pars intercerebralis major peptide-D2v) from the insect Locusta migratoria by forming a complex with the enzyme.

Ações bioquímicas/fisiológicas

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α₁-antitrypsin, α₂-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Definição da unidade

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Nota de preparo

TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity.

Nota de análise

Protein determined by A1%/280

Outras notas

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Informações de segurança

• Pictogramas: GHS08,GHS07,GHS09
• Palavra indicadora: Danger
• Frases de perigo: H302,H315,H319,H334,H335,H400
• Declarações de precaução: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Faceshields, Gloves