C9584

Carboxypeptidase B from porcine pancreas

lyophilized powder

• Sinônimo(s): Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase
• CAS Number: 9025-24-5
• Número CE: 3.4.17.2 (BRENDA, IUBMB)
• Número CE: 232-788-9
• Número MDL: MFCD00081476
• Código UNSPSC: 12352204
• ID de substância PubChem: 24893175
• NACRES: NA.54

Propriedades

• fonte biológica: Porcine pancreas
• Nível de qualidade: 200
• grau: Proteomics Grade
• Formulário: lyophilized powder
• atividade específica: ≥125 units/mg protein
• peso molecular: 34,000 Da± 600
• purificado por: affinity chromatography
• composição: Protein, 40-70%
• atividade externa: carboxypeptidase A ≤1%; chymotrypsin ≤0.1%; trypsin ≤5%
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
• InChI: 1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
• chave InChI: TWURVFFNODFJBJ-UHFFFAOYSA-N

Descrição

Aplicação

Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.

The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.

Ações bioquímicas/fisiológicas

Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.

Embalagem

Package size based on protein content

Definição da unidade

One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.

forma física

Contains HEPES buffer salts, zinc chloride and carbohydrate

Nota de preparo

Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

Nota de análise

Protein determined by biuret.

Informações de segurança

• Pictogramas: GHS08,GHS07
• Palavra indicadora: Danger
• Frases de perigo: H315,H319,H334,H335
• Declarações de precaução: P302 + P352 - P305 + P351 + P338
• Classificações de perigo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órgãos-alvo: Respiratory system
• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves