G1549

PNGase F

ready-to-use solution, recombinant, expressed in E. coli

• Sinônimo(s): N-glicosidase F, PNGase F, Peptídeo N-glicosidase
• CAS Number: 83534-39-8
• Número CE: 3.5.1.52 (BRENDA, IUBMB)
• Número MDL: MFCD18839214
• Código UNSPSC: 12352204
• NACRES: NA.54

Propriedades

• recombinante: expressed in E. coli
• Nível de qualidade: 200
• conjugado: (N-linked)
• grau: Proteomics Grade
• Formulário: ready-to-use solution
• atividade específica: ≥1000 U/mg
• prazo de validade: ≥1 yr at -20 °C
• peso molecular: ~36 kDa
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C

Descrição

Aplicação

Usado para desglicosilar proteína.

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.

Ações bioquímicas/fisiológicas

Cliva um glicano inteiro de uma glicoproteína, desde que a porção de asparagina glicosilada seja substituída em suas terminações amina e carboxila com uma cadeia polipeptídica.

PNGase F from Elizabethkingia meningoseptica has glycan-binding catalytic domain and a bowl-like domain at the N-terminus. It cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It is cost-effectively produced on a large scale in prokaryotic hosts and requires divalent zinc ions for its enzymatic activity.

Definição da unidade

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

forma física

Supplied as 300 Units/mL enzyme in 50% (v/v) glycerol and 50% (v/v) 20 mM Potassium Phosphate, pH 7.5.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable