L6397

Concanavalin A from Canavalia ensiformis (Jack bean)

peroxidase conjugate, lyophilized powder

• Sinônimo(s): ConA
• Código UNSPSC: 12352202
• NACRES: NA.32

Propriedades

• fonte biológica: Canavalia ensiformis (jack bean)
• Nível de qualidade: 200
• conjugado: peroxidase conjugate
• Formulário: lyophilized powder
• técnica(s): blood typing: suitable; fractionation: suitable
• Impurezas: carbohydrate, essentially free
• temperatura de armazenamento: −20°C

Descrição

Aplicação

Detects glycoproteins containing α-D-mannose, α-D-glucose when used with appropriate peroxidase substrate

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

Ações bioquímicas/fisiológicas

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Embalagem

Package size based on protein content

Definição da unidade

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

forma física

Lyophilized powder containing tris-citrate buffer salts and trace calcium and manganese

Nota de preparo

Prepared from peroxidase type VI (P8375), using the method of Wilson and Nakane, which promotes conjugation but prevents the interaction between Con A and peroxidase sugar residues.

Nota de análise

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H317,H334,H361
• Declarações de precaução: P202 - P261 - P272 - P280 - P302 + P352 - P308 + P313
• Classificações de perigo: Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges