700146P

alkyne-cholesterol

27-norcholest-5-en-25-yn-3β-ol, powder

• Synonym(s): alkynyl cholesterol
• Empirical Formula (Hill Notation): C₂₆H₄₀O
• CAS Number: 1631985-09-5
• Molecular Weight: 368.60
• MDL number: MFCD32706892
• UNSPSC Code: 12352211
• NACRES: NA.25

Properties

• Assay: >99% (TLC)
• form: powder
• packaging: pkg of 1 × 100 μg (700146P-100ug); pkg of 1 × 250 μg (700146P-250ug)
• manufacturer/tradename: Avanti Research^™ - A Croda Brand 700146P
• lipid type: click reagents
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Alkyne-cholesterol is a clickable cholesterol probe proprietary for Avanti Polar Lipids. They differ from cholesterol by having an alkyne group.

This reagent is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry′, in the presence of a copper (Cu)-containing catalyst.

Biochem/physiol Actions

Alkyne-cholesterol is not applicable for use in living cells due to copper ion (Cu (I) and Cu (II)) requirements. It aids in easy and sensitive detection of cholesterol using various biophysical methods. Alkyne-cholesterol can be conjugated to boron-dipyrromethene (BODIPY) fluorescent dye. It is useful in cholesterol trafficking studies in various organelles including mitochondria, endoplasmic reticulum and Golgi.

Packaging

5 mL Amber Glass Screw Cap Vial (700146P-100ug)

5 mL Amber Glass Screw Cap Vial (700146P-250ug)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable