203389

Blebbistatin, Racemic

≥97% (HPLC), liquid, Myosin II inhibitor, Calbiochem^®

• Synonym(s): InSolutionBlebbistatin, Racemic
• Empirical Formula (Hill Notation): C₁₈H₁₆N₂O₂
• Molecular Weight: 292.33
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Product Name: Blebbistatin, Racemic, InSolution, ≥97%, 50 mM in 90% DMSO, reversible inhibitor of nonmuscle myosin II
• Quality Level: 100
• Assay: ≥97% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A cell-permeable compound that acts as a selective, potent, and reversible inhibitor of non-muscle myosin II. Inhibits the ATPase and gliding motility of human platelets (≤100 µM) without affecting myosin light chain kinase (MLCK) activity. Has been shown to block cell blebbing and to rapidly disrupt directed cell migration and cytokinesis in vertebrate cells. Does not disrupt mitosis or affect contractile ring assembly.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
ATPase

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: <100 µM inhibiting the ATPase and gliding motility of human platelets

Packaging

Packaged under inert gas

Warning

Toxicity: Harmful & Carcinogenic / Teratogenic (E)

Physical form

A 50 mM (5 mg/342 µl) solution of (±)-Blebbistatin (Cat. No. 203390) in 90% DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Shu, S., et al. 2005. Proc. Natl. Acad. Sci. USA102, 1472.
Kovacs, M., et al. 2004. J. Biol. Chem.279, 35557.
Straight, A.F., et al. 2003. Science299, 1743.
Cheung, A., et al. 2001. Mol. Biol. Cell Suppl.12, 271a.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flash Point(C): 87 °C - closed cup - (Dimethylsulfoxide)