341631

Bovine Fibronectin

from bovine plasma, liquid, suitable for cell culture

• Synonym(s): Fibronectin, Bovine Plasma
• CAS Number: 86088-83-7
• MDL number: MFCD08705327
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• Product Name: Fibronectin, Bovine Plasma,
• description: Merck USA index - 14, 4075
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Fibronectin is an extracellular matrix (ECM) glycoprotein. It is expressed in various cell types. Fibronectin exists as a dimer and is made up of identical subunits which are linked covalently via a disulfide bond at their C-termini.

Application

Fibronectin, Bovine Plasma has been used as an extracellular matrix (ECM) substrate to coat dishes or polyacrylamide hydrogels for adhering:

• human melanoma cancer cells
• human breast epithelial cells
• mouse embryonic fibroblasts

Biochem/physiol Actions

Fibronectin plays a role in cell adhesion, growth, migration, and differentiation. It binds to several molecules such as integrins, heparin, fibrin, and collagen.

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

single band by SDS-PAGE

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable