527460

PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb

liquid, Calbiochem^®

• Synonym(s): Anti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG
• shipped in: wet ice
• storage temp.: −70°C
• target post-translational modification: phosphorylation (pThr451)
• Gene Information: human ... EIF2AK2(5610)

Description

General description

Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr⁴⁵¹.

Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr⁴⁵¹.

This PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)

Application

Immunoblotting (1:1000)

Immunohistochemistry (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

In Dulbecco′s PBS (without Mg⁺² or Ca⁺²), 0.1% BSA, 50% glycerol, pH 7.3.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Analysis Note

Positive Control
IFNγ-stimulated HeLa cells.

Other Notes

Cuddihy, A.R., et al.1999. Oncogene8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.

When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable