Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

70850

Sigma-Aldrich

FastPlax Titer Kit - Novagen

Rapid, reliable titering of baculovirus stocks

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352200
NACRES:
NA.54

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze

General description

The FastPlax Titer Kit is designed for determination of baculovirus titers in just 24 to 48 hours instead of three to four days as in other methods. Classical determination of baculovirus titer has relied on the appearance of plaques, but the FastPlax Kit takes advantage of the appearance of detectable levels of AcNPV gp64 glycoprotein on the cell surface as early as 8 to 24 hours postinfection. Detection is based on a high affinity monoclonal antibody against gp64, which is added directly to fixed cells. Antibody binding is detected with Goat Anti-Mouse IgG β-Galactosidase Conjugate followed by enhanced color development with X-Gal/NBT substrates. Plaques are clearly distinguished by their dark blue color. The kit contains sufficient reagents to perform five 6-well plate assays.

Packaging

Plastic ampoule

Components

  • 2 × 50 ml- 10X TBST (100 mM Tris-HCl, pH 8.0, 1.5 M NaCl, 1% Tween® 20)
  • 6 ml- 10% Gelatin (contains 0.02% azide)
  • 10 µl- FastPlax Antibody
  • 300 µl- Goat-Anti-Mouse β-Galactosidase Conjugate
  • 300 µl- NBT (83 mg/ml in 70% dimethylformamide)
  • 300 µl- X-gal (50 mg/ml in dimethylformamide)

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS

Storage and Stability

Multiple storage requirements

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service