MAB3516

Anti-Heat Shock Protein 70 Antibody, clone W27

clone W27, Chemicon^®, from mouse

• Synonym(s): HSP70
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: W27, monoclonal
• species reactivity: bovine, human, monkey
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2a
• NCBI accession no.: NM_002154.3; NM_198431.1
• UniProt accession no.: P34932
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HSPA4(3308)

Description

Specificity

Reacts with both the constitutive (HSP73) and inducible (HSP72) forms of HSP70.

POSITIVE CONTROL:

HeLa cells, BT474 cells or breast carcinoma.

Immunogen

HSP70 from HeLa cells.

Application

Immunoblotting: 0.5-1 μg/mL

Immunohistochemistry (frozen and formalin/paraffin): 2-4 μg/mL for 2 hours at room temperature. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Immunoprecpitation: 2 μg/mg protein lysate (use Protein G)

Flow cytometry

Optimal working dilutions must be determined by end user.

This Anti-Heat Shock Protein 70 Antibody, clone W27 is validated for use in IP, WB, IH(P) for the detection of Heat Shock Protein 70.

Physical form

Format: Purified

Analysis Note

Control
POSITIVE CONTROL: BT474, MAD109, RenCa, SK-NEP-1, U937 cells or breast carcinoma.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable