Skip to Content
MilliporeSigma

Skip To

MABE290

Sigma-Aldrich

Anti-Estrogen Receptor β Antibody, clone 9.88

clone 9.88, from mouse

Synonym(s):

Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2

Sign Into View Organizational & Contract Pricing

Select a Size

50 MG
MYR 1,120.00

MYR 1,120.00


Check Cart for Availability

Select a Size

Change View
50 MG
MYR 1,120.00

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

MYR 1,120.00


Check Cart for Availability

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9.88, monoclonal

species reactivity

human, mouse, rat

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

General description

Estrogen receptor beta (ERβ; NR3A2 or ESR2) is a nuclear steroid receptor that has been detected in the ovary, testis, thymus, spleen, bone, brain, and kidney. It is a multidomain protein containing A/B, C, D, E, and F domains, and it is activated by estrogen, resulting in homo- or heterodimerization (with ERα). The active ERβ-estrogen complex binds to regulatory regions known as estrogen response elements on target genes; however, ERβ may also regulate transcription without a bound ligand via its activation function (AF-1) region in the A/B domain. The ERβ receptor interacts with a wide variety of proteins including transcriptional corepressors and coactivators that regulate its activity. Its expression is also regulated by hypermethylation of CpG islands in the ERβ promoter. Previous studies have reported that ERβ has anti-inflammatory and anti-proliferative effects, and may therefore be an important therapeutic target for cancers, especially those occurring in the reproductive organs.

Immunogen

Recombinant protein corresponding to human Estrogen Receptor β.

Application

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Estrogen Receptor β in HUVEC cells (Simoncini, T., et al. (2006). Mol Endocrinol. 20(8):1756-1771.).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-Estrogen Receptor β Antibody, clone 9.88 (Mouse Monoclonal Antibody) validated in WB, IP to detect Estrogen Receptor β also known as Estrogen receptor beta.

Quality

Evaluated by Western Blot in PC-12 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Estrogen Receptor β in PC-12 cell lysate.

Target description

~55 kDa observed. Uniprot describes eight different isoforms ranging from ~36 kDa to ~59 kDa

Physical form

Format: Purified
Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
PC-12 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


  • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Select a Product Number

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Julio César Tobón-Velasco et al.
    Free radical biology & medicine, 53(5), 1024-1040 (2012-07-12)
    Pharmacological activation at the basal ganglia of the transcription factor Nrf2, guardian of redox homeostasis, holds a strong promise for the slow progression of Parkinson's disease (PD). However, a potent Nrf2 activator in the brain still must be found. In
    Mei-chin Mong et al.
    Journal of agricultural and food chemistry, 60(12), 3158-3165 (2012-03-08)
    Renal protection of s-allyl cysteine (SAC) and s-propyl cysteine (SPC) in diabetic mice against inflammatory injury was examined. Each agent at 0.5 and 1 g/L was added to the drinking water for 10 weeks. SAC or SPC intake significantly reduced
    Kevin T P Ng et al.
    PloS one, 7(2), e31655-e31655 (2012-03-06)
    Hepatocellular carcinoma (HCC) is highly malignant and metastatic. Currently, there is no effective chemotherapy for patients with advanced HCC leading to an urgent need to seek for novel therapeutic options. We aimed to investigate the effect of a garlic derivative
    Bioactive Nutraceuticals and Dietary Supplements in Neurological and Brain Disease: Prevention and Therapy (2014)
    Patricia Rojas et al.
    The Journal of nutritional biochemistry, 22(10), 937-944 (2010-12-31)
    S-Allylcysteine (SAC), the most abundant organosulfur compound in aged garlic extract, has multifunctional activity via different mechanisms and neuroprotective effects that are exerted probably via its antioxidant or free radical scavenger action. The 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mouse has been the most widely

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service