MABF2698

Anti-P. aeruginosa serogroup It-5 Antibody, clone PTA-5385

• Synonym(s): Pseudomonas aeruginosa It-5 serotype
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: PTA-5385, monoclonal
• mol wt: calculated mol wt N/A kDa; observed mol wt ~N/A kDa
• purified by: using protein G
• species reactivity: bacteria
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; inhibition assay: suitable
• isotype: IgG2κ
• epitope sequence: Unknown
• Protein ID accession no.: N/A
• UniProt accession no.: N/A
• storage temp.: -10 to -25°C

Description

Specificity

Clone PTA-5385 is a mouse monoclonal antibody that detects lipopolysaccharide (LPS) from It-5 serotype of Pseudomonas aeruginosa.

Immunogen

Heat killed Pseudomonas aeruginosa of It-5 serotype.

Application

Quality Control Testing

Evaluated by ELISA with LPS specific for serogroup It-5.

ELISA Analysis: Serial dilutions of this antibody detected LPS from Pseudomonas aeruginosa serogroup It-5.

Tested Applications

ELISA Analysis: A representative lot detected LPS from Pseudomonas aeruginosa (It-5) in ELISA applications (Lai, Z., et al. (2005). Vaccine. 23(25):3264-71).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Pseudomonas aeruginosa is a gram-negative, aerobic, non-spore forming bacteria that causes a variety of infections, particularly in immunocompromised subjects. Following infection, the airway mucosal immune system rapidly develops a pro-inflammatory response, leading to production of inflammatory mediators both in aerial compartment and local tissue initiated by the recognition of pathogen-associated molecular patterns (PAMPs) by specific Toll-like receptors and triggered by the epithelial cells and the resident immune cells. Pathogenesis is mediated by several virulence factors, including motility, adherence to biotic and abiotic surfaces, and secreted toxins. These factors modulate or disrupt host cells signaling pathways, target extracellular matrix, and induce tissue damage. Mouse monoclonal antibody, clone PTA-5385, developed against serotype It-5 directed to LPS O-side chain is opsonic for uptake and killing of bacteria by PMN and is shown to protect against fatal sepsis in mice rendered neutropenic with cyclophosphamide and challenged with homologous P. aeruginosa. (Ref.: Secher T., et al. (2011). J. Antimicrob. Chemother. 66(5); 1100 1109; Lai, Z., et al. (2005). Vaccine. 23(25); 3264-3271).

Physical form

Purified mouse monoclonal antibody IgG2 in PBS without preservatives.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable