MABT207

Anti-Integrin αV (ITGAV/CD51) Antibody

mouse monoclonal, 272-17E6

• Synonym(s): Integrin alpha-V, Vitronectin receptor subunit alpha, CD51, Integrin alpha-V heavy chain, Integrin alpha-V light chain, Intergrin av
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• Product Name: Anti-Integrin alpha-V Antibody, clone 272-17E6 (Azide free), clone 272-17E6, from mouse
• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 272-17E6, monoclonal
• species reactivity: human
• technique(s): activity assay: suitable; flow cytometry: suitable; immunoprecipitation (IP): suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001138472
• UniProt accession no.: P06756
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ITGAV(3685)

Description

General description

Integrin alpha-V (or CD51; vitronectin receptor subunit alpha) protein is a transmembrane receptor that mediates adhesion of cells to each other and to the extracellular matrix. Integrin receptors are activated by a variety of intracellular signals that modify its cytoplasmic domain and induce configural changes in integrin molecules thereby enabling ligand binding to the extracellular surface. An important part of this process is the binding of the actin protein, talin, to the integrin cytoplasmic domain.

Immunogen

Human Placental ɑvβ3

Application

Anti-Integrin alpha-V Antibody, clone 272-17E6 (Azide free) detects level of Integrin alpha-V & has been published & validated for use in Flow Cytometry, Immunoprecipitation, Activity Assay.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect Integrin alpha-V in M21 cell lysates. (Mitjans, F., et al (1995). Journal of Cell Science. 108:2825-2838.)

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 0.5 µg of this antibody detected Integrin alpha-V in HeLa cells.

Target description

116 kDa calculated

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservative.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
HeLa cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable