NA81

Anti-5-Methylcytosine Mouse mAb (162 33 D3)

liquid, clone 162 33 D3, Calbiochem^®

• Synonym(s): Anti-5-mc, Anti-5-MeCyd
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: 162 33 D3, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity (predicted by homology): all
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoaffinity purified mouse monoclonal antibody. Recognizes 5-methylcytosine.

Recognizes 5-methylcytosine in methylated DNA or RNA in NIH3T3 cells.

Specific
• Affinity purified mouse monoclonal antibody - Detects methylated DNA from a broad range of species. Several Applications
• Flow Cytometry
• Frozen Sections
• Immunoblotting
• Immunofluorescence
• Paraffin Sections

Reliable
• Generates reproducible results.
• Clone 162 33 D3 has been used in over 50 publications.

This Anti-5-Methylcytosine Mouse mAb (162 33 D3) is validated for use in ELISA, FC, Frozen Sections, IF, Paraffin Sections, Radioimmunoassay, Southwestern Blot for the detection of 5-Methylcytosine.

Immunogen

5-methylcytosine conjugated to ovalbumin

Application

ELISA (0.1 g/ml)
Flow Cytometry (1 g/ml)
Frozen Sections (5-10 g/ml)
Immunoblotting (5 g/ml)
Immunofluorescence (1 g/ml)
Paraffin Sections (5-10 g/ml)
Radioimmunoassay (0.1 g/ml)
Southwestern Blot (5 g/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
NIH3T3 cells

Other Notes

Clone 162 33 D3 is useful for the quantitative and qualitative detection of methylated DNA or RNA in a variety of samples and applications. It has been used in over 40 publications. Antibody should be titrated for optimal results in an individual systems.

Ehrlich, M. 2002. Oncogene21, 5400. (Review)
Widschwendter, M. and Jones, P.A. 2002. Oncogene21, 5462. (Review)
Barton, S.C., et al. 2001. Hum. Mol. Genet.10, 2983.
Piyathilake, C.J., et al. 2001. Hum. Pathol.32, 856.
Taddei, A., et al. 2001. Nat. Cell Biol.3, 114.
Piyathilake, C.J., et al. 2000. Biotech. Histochem.75, 251.
De Capoa, A., et al. 1999. FASEB J.13, 89.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable