HKG6PDH-RO

Hexokinase/Glucose-6-Phosphate Dehydrogenase (HK/G6P-DH)

from yeast/Leuconostoc, overproducer

• Synonym(s): G6P-DH, Glucose-6-Phosphate DehydrogenaseHK, Hexokinase
• UNSPSC Code: 12352204

Properties

• biological source: yeast
• Quality Level: 100
• form: suspension
• mol wt: dimer 110,000 Da
• packaging: pkg of 10 mL (10737275001 [30 mg]); pkg of 5 mL (10127825001 [15 mg])
• manufacturer/tradename: Roche
• concentration: 3.21 mg/mL
• technique(s): activity assay: suitable
• color: white
• pH: 5.5-6.5
• solubility: water: miscible
• suitability: suitable for UV spectrophotometry and general use
• application(s): life science and biopharma
• storage temp.: 2-8°C

Description

General description

Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH.

Application

Hexokinase/Glucose-6-phosphate dehydrogenase has been used for the determination of plasma glucose levels.

Components

EC 2.7.1.1 & 1.1.1.49

Unit Definition

One unit (U) HK will phosphorylate 1 μmol of D-glucose in one minute at +25 °C and pH 7.6.
One unit (U) G6P-DH will oxidize 1 μmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.
The coupled assay produces 1 μmol of NADH per μmol of D-glucose phosphorylated.

Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content.

Other Notes

For life science research only. Not for use in diagnostic prodcedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash