RNASEA-RO

RNase A

from bovine pancreas

• Synonym(s): Rnase
• EC Number: 3.1.27.5 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bovine pancreas
• Quality Level: 100
• form: dry powder
• specific activity: 50 U/mg (+25°C, RNA as substrate)
• mol wt: M_r = 13,700
• packaging: pkg of 100 mg (10109169001); pkg of 25 mg (10109142001)
• manufacturer/tradename: Roche
• parameter: 65 °C optimum reaction temp.
• technique(s): flow cytometry: suitable
• optimum pH: 7.0-7.5
• storage temp.: 2-8°C

Description

General description

Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins, and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form, RNase A exists as a homodimer.

Application

• For analytical purposes
• Isolation of DNA (for this purpose, RNase A should be boiled)
• For cell cycle analysis by flow cytometry and propidium iodide (PI) staining

Biochem/physiol Actions

RNase A catalyzes the transphosphorylation and degradation of RNA. It was the first protein identified to exhibit DNA melting functionality. This enzyme binds to several binding sites on single stranded (ss) RNA polynucleotide chain before degrading it. It interacts with ssDNA in a similar manner, which is responsible for its ‘DNA unwinding′ property. RNase A dimer obtained through tandemization shows toxicity to cancer cells.

Unit Definition

One unit is the enzyme activity that causes a decrease in absorbance of A₀ to A₁ within 1 minute under assay conditions. A₀ to A₁ corresponds to the total conversion, A₁ being the final absorbance.

Reconstitution

RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to 100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend into aliquots. Roche recommends subsequent storage at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable