77515

Methylene Blue solution

• Synonym(s): Ehrlich’s reagent III, Loeffler’s Methylene Blue
• Empirical Formula (Hill Notation): C₁₆H₁₈ClN₃S
• CAS Number: 61-73-4
• Molecular Weight: 319.85
• Beilstein: 3641570
• MDL number: MFCD00012111
• UNSPSC Code: 12171500
• PubChem Substance ID: 329767738
• NACRES: NA.47

Properties

• form: solution
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbe id | staining: suitable
• suitability: suitable for microscopy; Corynebacterium spp.; bacteria; mycobacteria; spore forming microorganisms
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChI key: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Description

Application

Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm.

Components

Composition:
methylene blue 0.3 g
ethanol 30 ml
water 100 ml

Other Notes

This product has been replaced by 03978-SIGMA | Methylene Blue solution for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 113.0 °F
• Flash Point(C): 45 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves