B8806

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester

1 mg/mL in DMSO

• Synonym(s): BCECF-AM solution, Spiro(isobenzofuran-1(3H),9′-(9H)xanthene)-2′,7′-dipropanoic acid
• EC Number: 200-664-3
• MDL number: MFCD00036969
• UNSPSC Code: 12171500
• NACRES: NA.47

Properties

• description: Mixture of six isomeric compounds
• Quality Level: 200
• form: liquid
• concentration: 1 mg/mL in DMSO
• color: colorless to yellow
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: CC(=O)OCOC(=O)CCc1cc2c(Oc3cc(OCOC(C)=O)c(CCC(=O)OCOC(C)=O)cc3C24OC(=O)c5cc(ccc45)C(=O)OCOC(C)=O)cc1OCOC(C)=O
• InChI: 1S/C42H40O21/c1-22(43)52-17-57-34-15-36-32(13-27(34)7-10-38(48)59-19-54-24(3)45)42(31-9-6-29(12-30(31)41(51)63-42)40(50)61-21-56-26(5)47)33-14-28(8-11-39(49)60-20-55-25(4)46)35(16-37(33)62-36)58-18-53-23(2)44/h6,9,12-16H,7-8,10-11,17-21H2,1-5H3
• InChI key: NTECHUXHORNEGZ-UHFFFAOYSA-N

Description

Application

2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester has been used for the estimation of vacuolar pH and intracellular pH.

Biochem/physiol Actions

An intracellular fluorescent pH indicator.

Hydrolyzed to BCECF by cytosolic esterases.

Physical form

Solution in 0.1% BCECF AM Ester and 99.9% DMSO

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 185.0 °F
• Flash Point(C): 85 °C