I5502

Isopropyl β-D-1-thiogalactopyranoside

≥99% (TLC)

• Synonym(s): IPTG, Isopropyl β-D-thiogalactoside
• Empirical Formula (Hill Notation): C₉H₁₈O₅S
• CAS Number: 367-93-1
• Molecular Weight: 238.30
• Beilstein: 4631
• EC Number: 206-703-0
• MDL number: MFCD00063273
• UNSPSC Code: 12352201
• PubChem Substance ID: 24896069
• NACRES: NA.31

Properties

• description: non-ionic
• Quality Level: 200; 300
• Assay: ≥99% (TLC)
• form: powder
• technique(s): thin layer chromatography (TLC): suitable
• impurities: 10.0-22.0% Dioxane
• color: white to off-white
• mp: 120 -122 °C ((248 - 252 °F ))
• solubility: water: 50 mg/mL, clear, colorless
• storage temp.: 2-8°C
• SMILES string: CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• InChI key: BPHPUYQFMNQIOC-NXRLNHOXSA-N

Description

Application

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Other Notes

Non-metabolizable galactose analog.

To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Reconstitution

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

Safety Information

• Pictograms: GHS07,GHS08,GHS07,GHS08
• Signal Word: WarningDanger
• Hazard Statements: H319 - H351 - H319 - H350
• Precautionary Statements: P281 - P202 - P264 - P280 - P305 + P351 + P338 - P305 + P351 + P338 - P308 + P313 - P337 + P313
• Hazard Classifications: Carc. 1B - Eye Irrit. 2
• Supplementary Hazards: EUH019
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable